Free Download MEDICAL BIOTECHNOLOGY by Glick et al

MEDICAL BIOTECHNOLOGY by Glick B.R. et al

Molecular biotechnology uses a variety of techniques for isolating genes and transferring them from one organism to another. At the root of these technologies is the ability to join a sequence of deoxyribonucleic acid (DNA) of interest to a vector that can then be introduced into a suitable host. This process is known as recombinant DNA technology or molecular cloning. A vast number of variations on this basic process has been devised. Development of the core technologies depended on an understanding of fundamental processes in molecular biology, bacterial genetics, and nucleic acid enzymology (Box 1.1). The beginning of the application of these technologies for the purpose of manipulating DNA has been credited to Stanley Cohen of Stanford University, Stanford, California, who was developing methods to transfer plasmids, small circular DNA molecules, into bacterial cells, and Herbert Boyer at the University of California at San Francisco, who was working with enzymes that cut DNA at specii c nucleotide sequences